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Satellite Mascot Workshop

13 May 2005
09:00am - 04:30pm

IMS Auditorium
Institute for Mathematical Sciences (NUS)
3, Prince George's Park, Singapore 118402

 Speaker

  • David Creasy (Matrix Science, UK)

 Schedule

Morning 09:00am - 12:30pm, MASCOT Users Workshop

  1. Mascot search overview
    Brief overview of the Mascot architecture and different ways of submitting searches / seeing results.

  2. Types of search
    The three main types of search: PMF, MS-MS and sequence tags will be described.

  3. Search parameters
    Optimising settings for:
      Database / Taxonomy
      Enzyme Tolerances
      Modifications

  4. Data file format
    The supported formats, including the new mzData format will be briefly described. Various tools for creating and combining peak list files will be shown.

  5. Results format
    The following results formats will be shown and described:
      Protein summary
      Concise protein summary
      Peptide summary
      Select summary
    Methods of drilling down into the results will be shown. Techniques for exporting results and sharing results with other users will be described.

  6. Interpreting results
    Understanding the Mascot score, significance thresholds and expectation values.
    Issues with large searches and 'MudPit' scoring.

  7. Error tolerant searching
    An example will be given, showing why and when it is useful and also how to interpret the results.

Afternoon 02:00pm - 04:30pm, MASCOT Administrators workshop

  1. Sequence database set up
    Setting up a 'standard' database. Automating downloads. Understanding what files are required, and where to find them.

  2. Installing Updates

  3. Installing and configuring Mascot Daemon
    Brief explanation of what it does. Description of the architecture, and the implications of the engine running as a service.
    Sharing the database between users

 Venue

  • IMS Auditorium
    Institute for Mathematical Sciences, NUS
    3, Prince George's Park
    Singapore 118402

 Registration

  • Registration Closed

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